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Deveopmental biology

Rescuing embryonic brains

Biologists rescue brains by re-engineering cell voltage patterns in embryos...

Tufts University biologists have demonstrated for the first time that electrical patterns in the developing embryo can be predicted, mapped, and manipulated to prevent defects caused by harmful substances such as nicotine. The research, published in Nature Communications, suggests that targeting bioelectric states may be a new treatment method for regenerative repair in brain development and disease, and that computational methods can be used to find effective repair strategies.

In a developing embryo, the outer membrane of each cell contains protein channels that transport negative and positive ions, generating voltage gradients across the cell wall. Groups of cells create patterns of membrane voltage that precede and control the expression of genes and the morphological changes occurring over the course of development.
"Studies focusing on gene expression, growth factors, and molecular pathways have provided us with a better but still incomplete understanding of how cells arrange themselves into complex organ systems in a growing embryo. We are now beginning to see how electrical patterns in the embryo are guiding large scale patterns of tissues, organs, and limbs. If we can decode this electrical communication between cells, then we might be able to use it to normalize development or support regeneration in the treatment of disease or injury."

Michael Levin PhD, Director and Professor, the Allen Discovery Center, Tufts University, and corresponding author of the study.

To help decipher that code, Levin and lead author Vaibhav Pai PhD, a Research Scientist II at the Allen Discovery Center at Tufts, explored whether it was possible to use a computational model to predict the bioelectrical patterns that occur in normal and nicotine-exposed embryos, and then use the model to identify reagents that might restore the normal pattern even in the presence of the teratogen (a class of molecules that induce birth defects). In humans, nicotine has been linked to prenatal morbidity, sudden infant death, attention deficit hypersensitivity disorder (ADHD), and other deficits in cognitive function, learning, and memory.
Earlier studies suggested that these defects may be a result of nicotine depolarizing cells in the embryo by inducing acetylcholine receptors to pump in positively charged sodium and potassium ions. Levin and Pai hypothesized that disruption of the normal bioelectric prepattern that drives brain patterning could be the underlying cause for these defects, and that restoring this pattern might rescue those defects.

Pai worked with Alexis Pietak PhD, a primary investigator at the Allen Discovery Center at Tufts, who developed a powerful computational simulation platform - called the BioElectric Tissue Simulation Engine (BETSE) - to create a dynamic map of voltage signatures in a developing frog embryo. The simulation engine (available for free download) was built on a biologically realistic model of ion concentrations and fluxes and parameters of ion channel behaviors derived from molecular studies.
The BETSE model accurately replicated the distinct pattern of membrane voltage from the normal embryonic brain development, and also explained the "flattened" (erased) electrical pattern observed to result from nicotine exposure.

The Tufts researchers were then able to use BETSE to explore the effect of various reagents on the embryo's voltage map. One reagent in particular, the hyperpolarization-activated cyclic nucleotide gated channel (HCN2), when added to the cells in the model, selectively enhanced hyperpolarization (large internal negative charge) in areas where it was diminished by nicotine. The effect - akin to dialing up the contrast in a photo editor - effectively restored the normal electrical pattern.
Remarkably, expressing HCN2 in live embryos rescued them from the effects of nicotine, restoring a normal bio-electric pattern, brain morphology, markers of gene expression, and near normal learning capacity in the grown tadpole.

"This is an important step providing a realistic model that bridges the molecular, cellular, bio-electrical, and anatomical scales of the developing embryo. Adding the bioelectrical component was critical to making a search for therapeutic strategies more tractable," adds Levin, the Vannevar Bush professor of biology in the School of Arts & Sciences at Tufts.

Endogenous bioelectrical signaling coordinates cell behaviors toward correct anatomical outcomes. Lack of a model explaining spatialized dynamics of bioelectric states has hindered the understanding of the etiology of some birth defects and the development of predictive interventions. Nicotine, a known neuroteratogen, induces serious defects in brain patterning and learning. Our bio-realistic computational model explains nicotine’s effects via the disruption of endogenous bioelectrical gradients and predicts that exogenous HCN2 ion channels would restore the endogenous bioelectric prepatterns necessary for brain patterning. Voltage mapping in vivo confirms these predictions, and exogenous expression of the HCN2 ion channel rescues nicotine-exposed embryos, resulting in normal brain morphology and molecular marker expression, with near-normal learning capacity. By combining molecular embryology, electrophysiology, and computational modeling, we delineate a biophysical mechanism of developmental brain damage and its functional rescue.

Authors: Vaibhav P. Pai, Alexis Pietak, Valerie Willocq, Bin Ye, Nian-Qing Shi and Michael Levin

Competing interests: The authors declare no competing interests.

The work was supported by The Paul G. Allen Frontiers Group, the W.M. Keck Foundation, the G. Harold and Leila Y. Mathers Charitable Foundation, the Templeton World Charity Foundation, the National Institutes of Health (AR055993-01, AR061988), and the American Heart Association. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

V.P.P. performed experiments and V.P.P. and M.L. designed the experiments and interpreted data. A.P. designed all models and executed the BETSE computational model, analysis, and interpretation. V.W. assisted with the geometric morphometric analysis. B.Y. performed the HCN2 electrophysiology experiments. N.Q.S. analyzed the HCN2 electrophysiology data and wrote the corresponding manuscript sections. V.P.P. and M.L. wrote the manuscript together, with contributions from A.P. About Tufts University: Tufts University, located on campuses in Boston, Medford/Somerville and Grafton, Massachusetts, and in Talloires, France, is recognized among the premier research universities in the United States. Tufts enjoys a global reputation for academic excellence and for the preparation of students as leaders in a wide range of professions. A growing number of innovative teaching and research initiatives span all Tufts campuses, and collaboration among the faculty and students in the undergraduate, graduate and professional programs across the university's schools is widely encouraged.

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Mar 12, 2018   Fetal Timeline   Maternal Timeline   News   News Archive

Normal frog embryo brain pattern development (left); frog embryo brain pattern when exposed to nicotine (center); frog embryo brain pattern rescued by HCN2 in the presence of nicotine (right)
Image credit: Vaibhav Pai (Tufts University Research Scientist)

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